Partek Flow Documentation

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Numbered figure captions
SubtitleTextRegion average coverage summary (truncated). Regions are on rows, samples on columns. The icon in the right-most column opens a coverage graph for that region
AnchorNamereg-coverage-summary

The browser icon in the right-most column (Image RemovedImage Added) of the Region average coverage summary table opens the Coverage graph for the respective region (Figure 5). The horizontal axis is the normalized position within the genomic feature, represented as 1st to 100th percentile of the length of the feature. The vertical axis is coverage. Each line on the plot is a single sample, and the samples are listed below the plot.

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  • Region name: the genomic feature identifier (as specified in the annotation file)

  • Chromosome: the chromosome of the genomic feature (or region)

  • Start: the start position of the genomic feature (1-based)

  • Stop: the stop position of the genomic feature (2-based, which means the stop position is exclusive)

  • Strand: the strand of the genomic feature

  • Total exon length: the length of the genomic feature

  • Reads: the total number of reads aligning to the genomic feature

  • % GC: the percentage of GC contents of those reads aligning to the genomic feature

  • % N: the percentage of ambiguous bases (N) of those reads aligning to the genomic feature

  • (n)x: the proportion of the genomic feature which is covered by at least n number of alignments. [Note: n is the coverage level that you specified when submitting Coverage report task, defaults are 1×, 20×, 100×]

  • Average coverage: the average sequencing depth across all bases in the genomic feature

  • Average quality: the average quality score across covered bases in the genomic feature

  • Image RemovedImage Added : the invokes the Coverage graph across the genomic feature, showing the current sample only (Figure 23) (or mouse over to get a preview of the plot)

  •  : the invokes the Chromosome view and browses to the genomic location

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