Page History
...
Similar to Cell Ranger - Gene Expression task, the first time user will be asked to create a Reference assembly. In Partek® Flow®, we will use Cell Ranger ARC 2.0.0 to create reference assembly for all 10x Genomics analysis pipelines. Please refer to our Cell Ranger - Gene Expression task manual on how to build or use Reference assembly.
| Numbered figure captions | ||||
|---|---|---|---|---|
| ||||
|
Clicking the big grey button of Create Cell Ranger ARC 2.0.0 reference would pop up a new window where lists the requirements that users need to fill in (Figure 3). To create the same reference genomes (2020-A) that are provided in Cell Ranger by default, the transcriptome annotations are respectively GENCODE v32 for human and vM23 for mouse, which are equivalent to Ensembl 98[3]. If users don't have any options in the dropdown list, they can click Add annotation model (GTF file) for Index, or New assembly... (FASTA file)for Assembly and upload the files.
| Numbered figure captions | ||||
|---|---|---|---|---|
| ||||
|
Once the right options Once the right assembly has been chosen/provided, simply press the Create button to finish. The reference assembly of ‘Homo sapiens (human) - hg38’ has been created as an example here (Figure 4).
...
Overview
Content Tools