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The main task menu will be refreshed as above (Figure 54) for gene expression data if references have been added. Users can go ahead click the Finish button to run the task as default. 

While for Feature Barcode data, there 's are more information needed besides reference assembly. An additional section of Protein has been added to the interface if Single cell gene expression + Cell surface protein has been selected for Feature Barcode data (Figure 5). Firstly, push the button Select data node and select the correct data for feature of antibody capture or protein in the newly popped-out window (top right, Figure 5). Then upload the feature reference file (.csv) prepared by users for their datasets. A Feature Reference CSV file declares the molecule structure and unique Feature Barcode sequence of each feature present in your experiment. It should include at least six columns: id, name, read, pattern, sequence and feature_type. An example of TotalSeq™-B Feature Reference CSV has been linked here. But for more details, please refer to 10x Genomics webpage[4].

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