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In order to know whether the data has been adapter-trimmed for microRNA data, we can look at the pre-alignment QA/QC of the raw data, specifically the read length distribution. If the read length distribution peaks at approximately 22-23 bases, this usually means the data has been adapter-trimmed. However, if you have a fixed length distribution, then very likely the data is not adapter-trimmed and you will need to get the adapter sequence from your vendor or service provider and use the Trim adapter function to trim away the adapter sequence.

Partek^® Flow^® software Partek Flow software wraps Cutadapt [1], a widely used tool for adapter trimming. It can be used to trim adapter sequences in nucleotide-space data as well as color-space data.

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