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To make a Pie chart, open a new Data Viewer session in Flow (Figure 1).

Select Pie chart from ‘Available plots’ on the left side and drag it to Add new plot in middle panel. Then select any data node to create a new empty Pie chart . Plot controls are in the panel on the left. The <Previous and Next> buttons enable you to switch between genomic features as they appear in the parent Feature list. The Group by option defines the grouping on the x-axis, with the drop-down list containing the data attributes as present in the Data tab. As previously mentioned, the dot colors are controlled by the Color by option. If you want to change a color, select the Customize colors hyperlink. The resulting dialog (Figure 2) will enable you to replace an existing color by a color of your choice (click on the arrow head Image Removed to invoke the color mixer) or add more colors (Add color).. 

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The Connect by option is particularly useful for dependent study designs, where you can highlight the samples based on the same biological source by the connecting lines. The example on Figure 3 depicts results of a study where each RNA sample was processed by both RNA-seq and gene array; the lines connect the same samples. Finally, the Show box plot turns on (or off) per-group box-and-whiskers (for each level of the Group by attribute).

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