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Partek Flow Documentation

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SubtitleTextData are represented as circles
AnchorNameData node

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  • Click the mRNA node

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SubtitleTextSelect a data node to open the context-sensitive task menu
AnchorNameInvoking task menu

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Pre-alignment QA/QC assesses the quality of the unaligned reads and will help us determine whether trimming or filtering is necessary. 

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SubtitleTextTasks are represented as rectangles
AnchorNameTask node

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  • Click the Pre-alignment QA/QC node

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SubtitleTextNavigating to the task report using the context-sensitive menu
AnchorNameContext sensitive menu

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  • Click Task report

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SubtitleTextViewing the pre-alignment QA/QC report
AnchorNamePre-alignment QA/QC report

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  • Click sample SSR592573 in the data table of the report to open its sample-level report

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SubtitleTextAverage base quality score per position for sample SRR592573
AnchorNameAverage base quality score per position

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A Phred score is a measure of base call accuracy with a higher score indicating greater accuracy.

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  • Click RNA-Seq 5-AZA to return to the Analyses tab (Figure 7)

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