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The data for this tutorial comes from Johnson et al. (Science 2007), which first described the ChIP-Seq technique.

This study mapped genomic binding sites for neuron-restrictive silencer factor (NRSF) transcription factor across the genome. There are two samples: an NRSF-enriched ChIP sample (chip.bam) and a control sample of input DNA without antibody immunoenrichment (mock.bam). The chip.bam file contains ~1.7 million mapped reads and the mock.bam file contains ~2.3 million mapped reads. These .bam files contain the aligned genomic locations and sequences of all mapped reads. This data set contains reads from a single-end (SE) library; the differences in processing paired-end (PE) reads will be discussed when applicable. 

Data for this tutorial can be downloaded from the Partek website using this link - ChIP-Seq tutorial data. To follow this tutorial, download the 2 .bam files and unzip them on your local computer using 7-zip, WinRAR, or by going to the Next Generation Sequencing tab after selecting Help then On-line Tutorials from the Partek Genomics Suite main toolbar. 

 

 a similar program. Because of the large size of the .bam files, we recommend saving them to a local drive instead of trying to access them on a network drive. The first time a .bam file is read by Partek Genomics Suite, the file will be sorted to allow for faster access. Therefore, you must have write permissions for the .bam files after download and on the file folder where they are stored. 

 

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