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• Right click column 6. Average Coverage and select Sort Descending from the menu
• Select Find Overlapping Genes from the Tools option in the command toolbar (Figure 1)
  
  

• Select Add a new column with the gene nearest to the region in the Find Overlapping Genes dialog (Figure 2)
• Select OKOK 
  
  

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Please note that it is recommended that you annotate with the same database as when you performed mRNA quantification.

• Select OKOK 
  
  

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• Select row 45 and Browse to location to show a region within an intron of UNC45B. This may be a novel exon (Figure 6) 
  
  

• Select row 10482 and Browse to location to show a region that starts 1 bp after CD82. This peak may represent an extended exon (Figure 7) 
  
  

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