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In this tutorial, you will analyze an RNA-Seq experiment using the Partek Genomics Suite software RNA-Seq workflow. The data used in this tutorial was generated from mRNA extracted from four diverse human tissues (skeletal muscle, brain, heart, and liver) from different donors and sequenced on the Illumina^® Genome Analyzer™. The single-end mRNA-Seq reads were mapped to the human genome (hg19), allowing up to two mismatches, using Partek ^® Flow ^® alignment and the default alignment options. The output files of Partek Flow are BAM files which can be imported directly into Partek Genomics Suite 7.0 software. BAM or SAM files from other alignment programs like ELAND (CASAVA), Bowtie, BWA, or TopHat are also supported. This same workflow will also work for aligned reads from any sequencing platform in the (aligned) BAM or SAM file formats.

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