Partek Flow Documentation

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A histogram is a plot that summarizes the underlying frequency of a set of data with the variable of interest on one axis and the frequency distribution of that variable in the other axis. In Partek Flow, histogram can be invoked on continuous or categorical variable.

Invoking a histogram

From a data viewer session, drag the histogram icon onto the data viewer canvas click on New plot > Bar chart (Figure 1).


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SubtitleTextDrag histogram plot (red rectangle) onto data viewer canvassSelect the Bar chart option from the New plot menu.
AnchorNameInvoke Histogram 1

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Upon dropping the histogram clinking on the canvas Bar chart menu, a dialogue opens up with the different data nodes sources that can be displayed on the histogram. Select your data node of interest and the content data (Figure 2).


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SubtitleTextSelect Normalized count the appropriate data node (red) and content data to display on the histogram plot
AnchorNamePlot datanode on histogram

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The first row in the data will be displayed by default in the histogram and in this case, it is the histogram of the expression values for the gene A1BG (Figure 3).

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SubtitleTextHistogram showing the distribution of AIBG expression (red)
AnchorNameDefault display on histogram

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Change the data displayed on the histogram by using the Configure > Axes menu and selecting the desired variable to display. Here the data displayed was switched to “Expressed genes” which is a continuous variable (Figure 4).


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SubtitleTextAdd attribute to Pie chart.
AnchorNamePie chart with samples

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Histogram showing the distribution of expressed genes variable (red rectangle)
AnchorNameHistogram of expressed genes

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Use the "Sort by" function to sort the plot. The default sorting is by Value on the x-axis and this default setting is sorted in ascending order. Users have the option to change that by changing the Default to value or frequency in the sort option (Figure 5)


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SubtitleTextExample Pie chart with samples.
AnchorNamePie chart with samples

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Sort by function can be by Value or Frequency (red)
AnchorNameSorting options for continuous variable

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Users can color the histograms by a categorical attribute using the Color by function (in red below). The bars were colored by the graph-based classifications in the example below (Figure 6).


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SubtitleTextThe mouseover example Pie chart.Histogram annotated by automatic classifications
AnchorNameThe mouseover Pie chart

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Configuration card (red rectangle in Figure 6) for Pie chart in Flow includes the options:

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Annotate histogram

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The bars in the histogram above were stacked. They can be unstacked using the Style menu as seen below in red (Figure 7).

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SubtitleTextConfiguration and splitted example of Pie chart.Unstacked bars in the histogram plot
AnchorNameConfiguration and splitted example of Pie chart

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Unstacking histogram

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Users also have the option to bin by either Count or Size. When binned by Count, the user specifies the number of bins for the data and the distribution is fit into the specified number of bins. Data below is binned by Count (Figure 8).


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SubtitleTextDifferent modes of Pie Chart.Histogram of expressed genes with number of bins specified as 10
AnchorNameDifferent modes of Pie Chart

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Image of expressed genes with bin Count of 5

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When binned by Size, the user specifies the number of items in the bin (size of a bin). This is used to calculate the number of bins required for the data. Data below is binned by Size (Figure 10).


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SubtitleTextSave image dialog (default settings)
AnchorNameSave image

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Histogram of expressed genes with size of bin specified as 75
AnchorNameValue binned by Size image

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Click the Save image button Image Added to save a PNG or SVG image to your computer. 

Click the Send to notebook button Image Added to send the image to a page in the Notebook. 


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Additional Assistance

If you need additional assistance, please visit our support page to submit a help ticket or find phone numbers for regional support.

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