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• Click on the legend title, drag and drop it on the color option on the UMAP plot:

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Now that we have plotted the clustering results we can start exploring them. We have identified markers for 13 clusters, with apparent spatial separation. The sample is human front cortex, thus we can expect our clusters to represent some of the major cell types found in the tissue (e.g. astrocytes, microglia, oligodendrocytes etc). Looking at the markers for Cluster 4, we can see there are a few known astrocyte markers: AQP4, AGT, FGFR3.

• Drag and drop each one of the 3 genes from the biomarkers table onto of the 'Green, Red, Blue' features of the spatial plot to visualize the in-situ expression:

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Zoom in to better observe the cell-level expression patterns. 

• Use the mouse wheel control to zoom in and out on the image

Let's classify the cells from cluster 4 as astrocytes:

• Click Select & Filter > Criteria, drag the Graph-based attribute on the Add criteria box and select only Cluster 4.
• Click Classify > Classify selection. Type 'Astrocytes' and Save. 
• Click Apply classifications, type 'Cell type' in the box and click Run.

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