PGS Documentation

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Discover de novo motifs

  • Select Motif discovery Discovery from the Peak Analysis section of the ChIP-Seq workflow
  • Select Discover de novo motifs
  • Select OK

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SubtitleTextViewing the binding site for NRSF. Use the blue arrows to cycle through views of all motif found (if there are more than one). Select Reverse to view the reverse complement sequence.
AnchorNameVisualizing a motif

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In this case, the motif finder discovered a motif in the NRSF-enriched regions that is 16 base pairs in length. The height of each position is the relative entropy (in bits) and indicates the importance of a base at a particular location in the binding site.

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SubtitleTextViewing the Motifs spreadsheet
AnchorNameMotif spreadsheet

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1. Counts gives the summed counts for each base call across all occurrences of the motif in the region list as {A, C, G, T} 

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SubtitleTextViewing the instances spreadsheet
AnchorNameInstances

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1-4. chromosome, start, stop, strand give the position

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SubtitleTextViewing the results of motif search
AnchorNameMotif Summary

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In the MotifSearch spreadsheet, each motif used in the motif search is shown. The columns detail the results of the search for each motif that was found in the reads. 

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Neuwald, A. F., Liu, J.S., & Lawrence, C.E. (1995). Gibbs motif sampling: detection of outer membrane repeats (Vol. 4). Protein Science.

 

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