Partek Flow Documentation

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A histogram is a plot that summarizes the underlying frequency of a set of data with the variable of interest on one axis and the frequency distribution of that variable in the other axis. In Partek Flow, histogram can be invoked on continuous or categorical variable.

Invoking a histogram

From a data viewer session, drag the histogram icon onto the data viewer canvas click on New plot > Bar chart (Figure 1).


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SubtitleTextDrag histogram plot (red rectangle) onto data viewer canvassSelect the Bar chart option from the New plot menu.
AnchorNameInvoke Histogram 1

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Upon dropping the histogram clinking on the canvas Bar chart menu, a dialogue opens up with the different data nodes sources that can be displayed on the histogram. Select your data node of interest and the content data (Figure 2).


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SubtitleTextSelect Normalized count the appropriate data node (red) and content data to display on the histogram plot
AnchorNamePlot datanode on histogram

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The first row in the data will be displayed by default in the histogram and in this case, it is the histogram of the expression values for the gene A1BG (Figure 3).

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SubtitleTextHistogram showing the distribution of AIBG expression (red)
AnchorNameDefault display on histogram

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Histogram of Continuous variables

Change the data displayed on the histogram by using the Configuration settings > Content > Data and Configure > Axes menu and selecting the desired variable to display. Here the data displayed was switched to “Expressed genes” which is a continuous variable (Figure 4).

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SubtitleTextHistogram showing the distribution of expressed genes variable (red rectangle)
AnchorNameHistogram of expressed genes

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Use the "Sort by" function to sort the plot. The default sorting is by Value on the x-axis and this default setting is sorted in ascending order. Users have the option to change that by changing the Default to value or frequency in the sort option (Figure 5)

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SubtitleTextSort by function can be by Value or Frequency (red)
AnchorNameSorting options for continuous variable

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Users can color the histograms by a categorical attribute using the Color by function (in red below). The bars were colored by the graph-based classifications in the example below (Figure 6).


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SubtitleTextHistogram of expressed genes sorted by Value in descending order (red)
AnchorNameImage of expressed genes sorted by Value and in descending order

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annotated by automatic classifications
AnchorNameAnnotate histogram

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The bars in the histogram above were stacked. They can be unstacked using the Style menu as seen below in red (Figure 7).


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SubtitleTextUnstacked bars in the histogram plot
AnchorNameUnstacking histogram

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Users also have the option to bin by either Count or Size. When binned by Count, the user specifies the number of bins for the data and the distribution is fit into the specified number of bins. Data below is binned by Count (Figure 78).


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SubtitleTextHistogram of expressed genes with number of bins specified as 510
AnchorNameImage of expressed genes with bin Count of 5

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When binned by Size, the user specifies the number of items in the bin (size of a bin). This is used to calculate the number of bins required for the data. Data below is binned by Size (Figure 810).


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SubtitleTextHistogram of expressed genes with size of bin specified as 1075
AnchorNameValue binned by Size image

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Histogram of Categorical variable

In the figure below, a categorical variable (Classifications) was selected to be displayed in the plot and sorted by frequency in ascending order (Figure 9).

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SubtitleTextHistogram of Classifications variable sorted by ascending order of Frequency
AnchorNameImage with Categorical data displayed on plot

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For categorical data, the user can select number of groups in the categorical variable to be binned together. In the figure below, the Classifications variable is binned into groups of 5 (Figure 10).

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SubtitleTextHistogram of Classifications variable with bin groups set as 5
AnchorNameBinning of Categorical variable

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SubtitleTextDifferent modes of Pie Chart.
AnchorNameDifferent modes of Pie Chart

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Once you are pleased with the appearance of the Pie plot, push Save image button to save it to the local machine or click Save button to save the Data Viewer. The resulting dialog (Figure 8) controls the Format,Size and Resolution of the image file. The image will be saved in your favorite format (.svg, .png and .pdf).

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SubtitleTextSave image dialog (default settings)
AnchorNameSave image

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Click the Save image button Image Added to save a PNG or SVG image to your computer. 

Click the Send to notebook button Image Added to send the image to a page in the Notebook. 


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