Partek Flow Documentation

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This method is also similar to the default method in the Drop-seq cookbookAlignment Cookbook (Macosko et al. 2015), which collapses UMI barcodes with a Hamming distance of 1. 

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Drop-seq Alignment Cookbook v1.2 Jan 2016, James Nemesh, Steve McCarroll’s lab, Harvard Medical School  http://mccarrolllab.com/wp-content/uploads/2016/03/Drop-seqAlignmentCookbookv1.2Jan2016.pdf

Macosko E, Basu A, Satija R, et al. Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets. Cell 2015; 161(5):1202-1214. https://doi.org/10.1016/j.cell.2015.05.002

 

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