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The GUI is simple and easy to understand. For each option, the grey colored description explains more details (Figure 2).  If the dataset working on is single cell RNA-Seq, no it doesn't need the Micro environment file is needed. However, if the dataset it is a spatial data, most likely you would like to provide the Micro environment file because of its spatial contents. By default, the value of 0.10 will be used as threshold to select which cells are used for the analysis in the cluster. However, the number could be adjusted manually or typed in directly.  Simply click the Finish button if you want to run the task as default.

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