Visualize the annotated image

With samples imported and annotated, we can begin analysis. 

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• Duplicate the plot by clicking the Duplicate plot button in the upper right controls (arrow 1)
• Open the Axes configuration option (arrow 2)
• Change the Sample on the duplicated image under Misc (arrow 3)

Modify Style

To modify the points on the image to show more of the histology use the Style configuration option. 

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• Click Save in the left panel and give the session an appropriate name

Modify Axes

• Click Axes in the left panel
• Toggle off Show lines for both the X & Y axis
• Toggle off Show title and Show axis for both the X & Y axis

Color by gene expression

• Click Style
• Click the blue circle node to the right of the Color by drop-down
• Select the Normalized counts node
• Choose to Color by Numeric triad
• Use the Green drop-down to select IL32, Red drop-down to select DES, and Blue drop-down to select PTGDS gene expression

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