Partek Flow Documentation

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Why can't I view PCA on Google Chrome and I am being pointed to WebGL instructions?

If you are using Google Chrome, some graphics card (particularly in older Mac laptops) are not supported and they affect the ability to run WebGL (needed to display PCA). You may wish to use a different browser (Mozilla Firefox) or you can try running Google Chrome while ignoring any blacklisted hardware:

  • For Mac, this is the command: /Applications/Google\ Chrome.app/Contents/MacOS/Google\ Chrome --ignore-gpu-blacklist
  • Here is a video of a workaround and also creating an automated tool in a Mac: https://customer.partek.com/tutorials/ChromeWebGLWorkaround.mp4 (02:55)
  • The app created in the video is available for download here (ChromeWebGL.app.zip). Unzip and make sure to exit Google Chrome complete before running the app.

I have two projects that I ran separately. I want to

have a bigger project that has all samples but I don't want to repeat the alignment

combine the two projects without repeating the alignment step. How can I combine the aligned reads?

Create a third project. Import two sets of BAM files.

Project 1 - In Data Tab, take note of your project output folder. Also note the aligner that you used. 

Project 2 - In Data Tab, take note of your project output folder. Also note the aligner that you used. 

Project 3 - Create this new project. Import Data > Automatically create samples from files > Find the project output folder of Project 1 (should be in Partek Flow server) > Find the folder for the aligner you used > within Within that folder should be the BAM files> files > Select the BAM files > Create sample. Repeat same process for Project 2.

Now you will have all samples as one data node (aligned reads) in Project 3. You can proceed with downstream analysis (normalization, differential gene expression, etc).

 

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