Join us for an event September 26!
How to Streamline RNA-Seq analysis and increase productivity—point, click, and done
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Full Webinar
Time: 1.
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This is a demonstration of ATAC-Seq data analysis starting with the import of raw sequencing .fastq files, followed by alignment to the reference genome, peak detection, and annotation. Comparing peaks between different groups with multiple replicates is introduced. Motif detection is performed on significant regions and interpretation of the biology by conducting KEGG pathway enrichment analysis on genes overlap regions.
- Import fastq files
- Attribute management
- QA/QC
- Alignment
- Detect enriched regions with MACS2
- Annotate regions
- Compare regions among different groups
- Search for motifs on significant regions
- KEGG enrichment analysis
Visualization: scatterplot, TSS plot, volcano plot, heatmap etc.
25 hours
This training webinar was recorded live.
Webinar Breakdown
Topics have been broken out into bite-size videos for your convenience.
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Agenda
Time: 1 minute
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Data Set Overview
Time: 1 minute
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Bulk RNA-Seq Analysis Pipeline
Time: 37 minutes
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ATAC-Seq Alignment
Time: 2.5 minutes
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Filter Alignment
Time: 3.5 minutes
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Peak Detection and Chromosome View
Time: 11 minutes
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Filter Peaks
Time: 3 minutes
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Peak Annotation
Time: 7.5 minutes
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Compare Peak Regions Across Samples
Time: 5 minutes
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Compare Peak Regions Across Different Groups
Time7 minutes
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Motif Detection
Time: 3 minutes
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RNA-Seq and ATAC-Seq Integration
Time: 10.5 minutes
Overview
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