A histogram is a plot that summarizes the underlying frequency of a set of data with the variable of interest on one axis and the frequency distribution of that variable in the other axis. In Partek Flow, histogram can be invoked on continuous or categorical variable.
Invoking a histogram
From a data viewer session, drag the histogram icon onto the data viewer canvas click on New plot > Bar chart (Figure 1).
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SubtitleText | Drag histogram plot (red rectangle) onto data viewer canvassSelect the Bar chart option from the New plot menu. |
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AnchorName | Invoke Histogram 1 |
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Upon dropping the histogram clinking on the canvas Bar chart menu, a dialogue opens up with the different data nodes sources that can be displayed on the histogram. Select your data node of interest and the content data (Figure 2).
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SubtitleText | Select Normalized count the appropriate data node (red) and content data to display on the histogram plot |
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AnchorName | Plot datanode on histogram |
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SubtitleText | Histogram showing the distribution of AIBG expression (red) |
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AnchorName | Default display on histogram |
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Histogram of Continuous variables
Change the data displayed on the histogram by using the Configuration settings > Content > Data and Configure > Axes menu and selecting the desired variable to display. Here the data displayed was switched to “Expressed genes” which is a continuous variable (Figure 4).
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SubtitleText | Sort by function can be by Value or Frequency (red) |
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AnchorName | Sorting options for continuous variable |
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The sort menu was changed to Value in the case below and user can now sort Value by either ascending or descending order. Here the Value of expressed genes is sorted by descending order (Figure 6).
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SubtitleText | Histogram of expressed genes sorted by Value in descending order (red) |
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AnchorName | Image of expressed genes sorted by Value and in descending order |
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Users can color the histograms by a categorical attribute using the Color by function (in red below). The bars were colored by the graph-based classifications in the example below (Figure 76).
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SubtitleText | Histogram annotated by graph-based automatic classifications |
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AnchorName | Annotate histogram |
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The bars in the histogram above were stacked. They can be unstacked using the Style function menu as seen below in red (Figure 87).
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SubtitleText | Unstacked bars in the histogram plot |
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AnchorName | Unstacking histogram |
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Users also have the option to bin by either Count or Size. When binned by Count, the user specifies the number of bins for the data and the distribution is fit into the specified number of bins. Data below is binned by Count (Figure 98).
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SubtitleText | Histogram of expressed genes with number of bins specified as 510 |
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AnchorName | Image of expressed genes with bin Count of 5 |
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When binned by Size, the user specifies the number of items in the bin (size of a bin). This is used to calculate the number of bins required for the data. Data below is binned by Size (Figure 10).
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SubtitleText | Histogram of expressed genes with size of bin specified as 1075 |
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AnchorName | Value binned by Size image |
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Additional Assistance
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