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Flow Documentation

Partek Flow Documentation

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  • Click  to view the Volcano plot 
  • In the Configuration card Open the Style icon on the left, expand the Size card and increase the change Size point size to 6
  • In Open the Configuration card Axes icon on the left , expand the Data card and change the Y-axis to FDR step up (Glioma vs Oligodendrocytes)
  • In the Configuration card on the left, expand the Significance card and change Open the Statistics icon and change the Significance of X threshold to -10 and 10 and the Y threshold to 0.001 
  • In the Selection card on the rightOpen the Select & Filter icon, set the Fold change thresholds to -10 and 10
  • In the Selection card on the right Select & Filter, click  to remove the P-value (Glioma vs Oligodendrocytes) selection rule. From the drop-down list, add FDR step up (Glioma vs Oligodendrocytes) as a selection rule and set the maximum to 0.001

This gives 291 significant differentially expressed genes Note these changes in the icon settings and volcano plot below (Figure 6).


Numbered figure captions
SubtitleTextPreviewing a filter by adjusting the size of the points, changing the Y-axis, adjusting the X & Y significance thresholds and changing the selection criteria
AnchorNameVolcano plot

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We can now recreate these conditions in the GSA report filter. 

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