Partek Flow Documentation

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  • Upload either the filtered contig_annotations.csv file alone or, if you have matching gene expression data, with the filtered count matrix per sample and give each sample a name. To add a sample use the Action. In the The example below which is using the default settings , and there is one sample with two files, one for V(D)J, and one for gene expression. Click Finish

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  • The example below shows the results from the Clonotype Frequency Plot task which is accessed by choosing to perform this task from the Single cell V(D)J node and will automatically open in the data viewer for modification. In this case, the barcode frequency is the number of clonotypes per cell because the barcode usually represents a single cell, so there are two cells that have clonotype5 and clonotype . Clonotype 5 is made of two compositions (a frequency of four for clonotype5 from the V(D)J node) with a Chain, V gene, D gene, and J gene as seen below the bars and by hovering. 

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