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• B cell isotypes are defined by the Chain and C gene. In the examples below, Chain and C gene are plotted by Barcode frequency using a Bar chart. On the top, no selection and filtering has been performed. On the second plot, the data has been selected and filtered by the heavy chain. By using Select & filter, certain criteria can be focused on. The third plot is further stacked to 100% and colored by V gene because the V regions do not change specificity during isotype switching. 

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