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• B cell isotypes are defined by the Chain and C gene. In the examples below, Chain and C gene are plotted by Barcode frequency using a Bar chart. On the top, no selection and filtering has been performed. On the bottom second plot, the data has been selected and filtered by the heavy chain. By using Select & filter, certain criteria can be focused on. The third plot is further colored by V gene because the V regions do not change specificity during isotype switching. 

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• Below, CDR3 abundance is plotted by barcode frequency and colored by Clonotype ID in the top Bar chart. In the bottom example, the plot is instead colored by Chain and other modifications, such as axis ticks and the number of groups per page, has been made. Note that the predicted CDR3 amino acid sequence is plotted here, but the predicted CDR3 nucleotide sequence (cdr3_nt) as well as information for other Complementarity-Determining Regions is also available. 

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