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• B cell isotypes are defined by Chain and C gene. In the example below, Chain and C gene are plotted by Barcode frequency. On the left, no selection and filtering has been performed. On the plot on the right, the heavy chain has been selected and filtered by in the data. 

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References

1. Tonegawa, S. Somatic generation of antibody diversity. Nature 302,575–581 (1983). https://doi.org/10.1038/302575a0
2. https://support.10xgenomics.com/single-cell-vdj/software/pipelines/latest/output/annotation#contig-annotation 
3. https://support.10xgenomics.com/single-cell-vdj/software/overview/welcome 
   
4. https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/7.0/advanced/h5_matrices
5. https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/7.0/output/matrices
6. https://support.10xgenomics.com/single-cell-vdj/software/pipelines/latest/algorithms/annotation#productive
7. https://support.10xgenomics.com/single-cell-vdj/software/pipelines/latest/using/vdj 
8. https://support.10xgenomics.com/single-cell-vdj/software/pipelines/latest/using/multi