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To visualize the results, we can generate a hierarchical clustering heat mapheatmap. 

• Click the green Filtered feature list produced by the Differential analysis filter task
• Click Exploratory analysis in the task menu
• Click Hierarchical clustering/heat mapheatmap

Using the hierarchical clustering options we can choose to include only cells from certain samples. We can also choose the order of cells on the heat map heatmap instead of clustering. Here, we will include only glioma cells and order the samples by sample name (Figure 7).  

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• Double click the green Hierarchical clustering node to open the heat mapheatmap

The heat map heatmap will appear black at first; the range from red to green with a black midpoint is set very wide because of a few outlier cells. We can adjust the range to make more subtle differences visible. 

• Set Low to -1.5
• Press Enter
• Set High to 1.5
• Press Enter

The heat map heatmap now shows clear patterns of red and green. 

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