Partek Flow Documentation

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Numbered figure captions
SubtitleTextData Quality report of Cell Ranger - ATAC task in Flow.
AnchorNameCell ranger task report





Other parameter to adjust (Figure 2):

Subsample percentileDownsample to preserve this fraction of reads

Users can click Configure to change the default settings In in Advanced options (Figure 42).

Include intronsCount reads mapping to intronic regions. This may improve sensitivity for samples with a significant amount of pre-mRNA molecules, such as nuclei.

Expected cells: Expected number of recovered cells. Default: 3,000 cells.

Force cells: Force pipeline to use this number of cells, bypassing the cell detection algorithm. Use this Override peak caller: To override the peak caller, users specify peaks to use in downstream analyses from supplied 3-column BED file. The supplied peaks file must be sorted by position and not contain overlapping peaks; comment lines beginning with `#` are allowed.

Force cellsDefine the top N barcodes with the most fragments overlapping peaks as cells and override the cell calling algorithm. N must be a positive integer <= 20,000. Use this option if the number of cells estimated by Cell Ranger -ATAC is not consistent with the barcode rank plot.

Memory limit (GB): Restricts Cell Ranger - Gene ExpressionATAC to use specified amount of memory (in GB) to execute pipeline stages. 

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