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Clicking the big grey button of Create Cell Ranger 6ARC 2.0.0 reference would pop up a new window where lists the three pre-built reference genomes for human(hg38), mouse(mm10) and the mix of two(hg38-mm10), respectively (requirements that users need to fill in (Figure 3). They are exactly To create the same reference genomes (2020-A) that are provided in Cell Ranger by default. In details, the transcriptome annotations are respectively GENCODE v32  for human and vM23 for mouse, which are equivalent to Ensembl 98[3]. References for other organisms currently are not available in Flow Cell ranger, and will be coming in the future.If users don't have options in the dropdown list, they can click Add annotation model (GTF file) for Index, or New assembly... (FASTA file)for Assembly and upload the files. 

Once the right reference options has been chosen/provided, simply press the Create button to finish. The reference assembly of ‘hg38Homo sapiens (human) - hg38’ has been selected created as an example here (Figure 4).

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