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Start by choosing a factor or interaction from the Factor drop-down list. The subgroups of the factor or interaction will be displayed in the left panel; click to select a subgroup name and move it to one of the panels on the right. The fold change calculation on the comparison will use the group in the top panel as numerator, and the group in the bottom panel as the denominator. Click on Add comparison button to add one comparison to the comparisons table. Note that multiple comparisons can be added to the specified model.
ANOVA advanced options
Click on the Configure to customize Advanced options (Figure 17)
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When an attribute is selected, pairwise comparisons of all the levels will be performed independently.
Click on Configure button in the Advanced options to configure normalization method and library types (Figure 19).
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