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The first step of GSA is to choose which attributes to include in the test (Figure 1). All sample attributes including numeric and categorical attributes are displayed in the dialog, so use the check button to select between them. An experiment with two attributes Cell type (with groups A and B)and Time (time points 0, 5, 10) is used as an example in this section. Image Removed
Figure 1:

Click Next to display the levels of each attribute to be selected for sub-group comparisons (contrasts).

To compare A vs. B, select A for Cell type on the top, B for Cell type on the bottom and click Add comparison. The specified comparison is added to the table below (Figure 2). Image Removed
Figure 2:

To compare Time point 5 vs. 0, select 5 for Time on the top, 0 for Time on the bottom, and click Add comparison (Figure 3). Image Removed
Figure 3:

To compare cell types at a certain time point, e.g. time point 5, select A and 5 on the top, and B and 5 on the bottom. Thereafter click Add comparison (Figure 4). Image Removed
Figure 4:

Multiple comparisons can be computed in one GSA run,; Figure 5 is showing the above three comparisons are added in the computation.

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