Partek Flow Documentation

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Once the samples have been uploaded, the Data tab will list each sample along with the Platform associated with each sample. If Affymetrix .CEL files were uploaded, it will also include the date that the array was scanned (Figure 2). This information may be helpful in assessing possible batch effects.

 

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PrefixFigure
SubtitleTextImported microarrays will automatically include Platform and, in some cases, Scan date in the Attributes page
AnchorNameMicroarray Project, Data Tab

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Once the microarray data has been uploaded, the Microarray intensity data node will appear in the Analyses tab. To convert this data to aligned reads, select the Microarray intensity data node and select the aligner you would like to use for conversion (Figure 3).

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Figure 3:  

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PrefixFigure
SubtitleTextConverting Microarray intensity to Aligned reads
AnchorNameMicroarray Data Node

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Selecting an aligner will bring up the Microarray Conversion options page (Figure 4). Under the Select probe sequence file section, make sure that the correct Chip name is specified. For non-custom arrays, these should be set automatically to the platform detected upon importing the array.

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