A Pie Chart is a type of graph that displays data in a circular graph. It gives you a snapshot of how a group is broken down into smaller pieces. Because the pieces of a Pie chart are proportional to the fraction of the whole in each category. In order to make a Pie chart, you must have a list of categorical variables (descriptions of your categories, like ‘cell type’) as well as numeric variables (e.g.,  cell numbers). In Partek^® Flow^®, the default numeric variable is the cell numbers.  Therefore, the Pie chart indicates the fraction of the whole cell numbers in each category.

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The chart title is based on the genomic feature (e.g. gene or transcript) that the plot was invoked on. The y-axis is scaled automatically, based on the range of the data, and the units correspond to the input units of the parent Feature list node, i.e. if the data were normalized using transcripts per million (TPM), the y-axis will be in TPM-normalised counts. Dots represent samples. Hovering the cursor over a sample invokes a popup balloon message shows sample ID and the respective expression value. The legend is in the upper right corner and is based on the data attribute specified under the Color by option (on the left).Select Pie chart from ‘Available plots’ on the left side and drag it to Add new plot in middle panel. Then select any data node to create a new empty Pie chart. 

Plot controls are in the panel on the left. The <Previous and Next> buttons enable you to switch between genomic features as they appear in the parent Feature list. The Group by option defines the grouping on the x-axis, with the drop-down list containing the data attributes as present in the Data tab. As previously mentioned, the dot colors are controlled by the Color by option. If you want to change a color, select the Customize colors hyperlink. The resulting dialog (Figure 2) will enable you to replace an existing color by a color of your choice (click on the arrow head to invoke the color mixer) or add more colors (Add color).

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