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• Click and drag the 2D scatter plot icon from the Available plots card onto the canvas (Figure ?)
• Drop the 2D scatter plot to the right of the UMAP plot

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• In the Selection card on the right, click Rule to change the selection mode
• Click the blue circle next to the Add rule drop-down menu (Figure ?)

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• Click Merged counts to change the data source for the drop-down list
• Choose CD3_TotalSeqB from the drop-down list (Figure ?)

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• Click Merged counts in the Data card on the left
• Click and drag CD8a_TotalSeqB onto the 2D scatter plot (Figure ?)
• Drop CD8_TotalSeqB onto the x-axis option

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