Partek Flow Documentation

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  • Feature ID information: if transcript level analysis was performed, and the annotation file has both transcript and gene level information, both gene ID and transcript ID are displayed. Otherwise, the table shows only the available information.
  • Total reads: total number of read across all the samples from the input data in linear space. If the input data is quantification output, then the total reads is the sum of reads of raw counts; if the input data is normalized, then the total reads is the sum of normalized reads; if the input data is logged, then the total reads is the sum of anti-logged value.
  • Each contrast outputs p-value, FDR step up p-value, ratio and fold change in linear scale, LSmean of each group comparison in linear scale

Click on View extra details report (Image Added) icon under View section to get more statistical information about the feature.  In a case that the task doesn't fail, but certain statistical information is not generated, e.g. p-value and/or fold change of a certain comparison are not generated for some or all feature, click on this icon to get more information by mousing over the read exclamation icon

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By clicking on Optional columns, you can retrieve more statistics result information, e.g. Average coverage which is the geometric mean of normalized reads in linear scale across all the samples; fold change lower/upper limits generated from 95% confidence interval;  feature annotation information if there are any more annotation fields in the annotation model you specified for quantification, like genomic location, strand information etc.


On the right of each contrast header, there is volcano plot icon (  ). Select it to display the volcano plot on the chosen contrast (Figure 11).

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