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• File formats for next generation sequencing data
• Phred-quality score

Showing Pre-alignment tools

In order to show the Pre-alignment tools, select an Unaligned reads or Trimmed reads data node. They will appear on the context-sensitive menu on the right of the screen (Figure 1).

Different Pre-alignment tools are available for different formats of unaligned reads. For example: if the reads are in FASTQ format, then all three tools are available.On the other hand, if the unaligned reads are in FASTA or SFF format, then the option Subsample FASTQ is not available.

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