Page History
...
- Choose Graph-based from the Select by drop-down in the Attributes tab of the Selection / Filtering section of the control panel
- Click the check box for 8 to select cluster 8
- Click Classify selection
- Name the cells Tfh cells
- Click Save
- Choose Classifications from the Color by drop-down menu
- Click Clear selection
- Click Clear filters to return to the full data set
- Click Apply classifications
Classify cells using Scatter plot
An alternative method to clustering and UMAP/t-SNE for classifying cells is using a scatter plot to visualize the expression of key marker genes or proteins. This approach is more effective with CITE-Seq data than gene expression data alone as the protein expression data has a better dynamic range and is less sparse.
- Click the Merged counts data node
- Click Exploratory analysis in the toolbox
- Click Scatter plot
- Click Finish to run
- Double-click the Scatter plot task node to open it
Overview
Content Tools