Partek Flow Documentation

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  • Choose Graph-based from the Select by drop-down in the Attributes tab of the Selection / Filtering section of the control panel 
  • Click the check box for to select cluster 8
  • Click Classify selection 
  • Name the cells Tfh cells
  • Click Save 
  • Choose Classifications from the Color by drop-down menu
  • Click Clear selection 
  • Click Clear filters to return to the full data set
  • Click Apply classifications 

Classify cells using Scatter plot

An alternative method to clustering and UMAP/t-SNE for classifying cells is using a scatter plot to visualize the expression of key marker genes or proteins. This approach is more effective with CITE-Seq data than gene expression data alone as the protein expression data has a better dynamic range and is less sparse. 

  • Click the Merged counts data node
  • Click Exploratory analysis in the toolbox
  • Click Scatter plot
  • Click Finish to run 
  • Double-click the Scatter plot task node to open it