When If your experimental design includes a sample or a group of samples serves serving as a baseline controlbaseline control, you can normalize the experimental samples can be normalized by subtracting or dividing by the baseline sample(s) , e.g. in PCR data using the Normalize to baseline task in Partek Flow. For example, in PCR experiments, the delta Ct values of control samples are subtracted from the delta Ct values of experimental samples to obtain delta delta CT values for the experimental samples. 

The Normalize to baseline option is available in the Normalization and Scaling section of the context-sensitive menu (Figure 1) upon selection of any count matrix data node.

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