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SubtitleText Changing column 1 properties AnchorName Properties of column dialog
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• Select OK

The samples names in column 1 are now black, indicating that they have been changed to a categorical variable. Next, we will add attributes for grouping the data. 

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• From the RNA-seq workflow panel, select Add sample attributeSample Attributes to bring up the Add Sample Attributes dialog (Figure 4)
  
  

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SubtitleText Add Sample Attributes dialog AnchorName Add sample attributes dialog

• Select Add a categorical attributeCategorical Attribute
• Select OK to bring up the Create categorical attribute dialog 

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SubtitleText Alignment_Counts spreadsheet AnchorName Alignment counts spreadsheet
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This spreadsheet shows that all reads mapped to one location or did not align to any location. This is dependent on the options used during import. For other data and alignment options, there might be reads with more than one alignment. Reads that were not aligned are listed here because BAM files contain all aligned and unaligned reads; however, unaligned reads are excluded from downstream analysis.  

 

 

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