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• Select Chromosomal Location under the Column Configuration panel (Figure 4). Leave everything else as default and select OK
  
  

Interestingly, of the 23 genes of the Down_Syndrome_vs_Normal (A) spreadsheet, 20 genes are located on chromosome 21. This suggests that the gene expression changes associated with Down syndrome observed in this study are primarily located on chromosome 21, not distributed throughout the genome, an important finding of this study. 

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