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• From the Gene Expression workflow, choose Biological Interpretation followed by Gene Set Analysis
• Select the GO Enrichment radio button in the Gene Set Analysis dialog (Figure 1) followed by Next
• Make In the next dialog, make sure the Gene_List.txt spreadsheet is chosen from the dropdown list and click Next

...

• Select the Default mapping file radio button and click Next
  

A new spreadsheet (Figure 3) and the gene ontology browser (Figure 4) will appear.

The new spreadsheet (GO Enrichement.txt) is a child spreadsheet of the gene list. The first column contains the GO functional group, each of which falls into the broader categories of biological process, cellular component or molecular processes shown in column 2. The GO functional groups are arranged by descending enrichment score, which is shown in the third column. The higher the enrichments score, the more over represented this functional group is in