This tutorial gives an overview of RNA-Seq analysis with Partek® Flow®. It will guide you through an RNA-Seq workflow and result in the creation of a list of differentially expressed genes and subsequent visualisation of gene signatures by hierarchical clustering and interpretation of the list by gene ontology (GO) enrichment.
Specifically, this tutorial will illustrate how to:
- Getting the Tutorial Data Set
- Annotating Samples with Attributes
- Performing Pre-alignment QA/QA
- Aligning Data to a Reference Genome
- Performing Post-alignment QA/QC
- Estimating Transcript Abundance
- Exploratory Analysis by PCA
- Normalizing Gene Counts
- Detecting Differentially Expressed Genes
- Creating a List of Significant Genes
- Visualizing the Gene List by Heat Map
- Performing Biological Interpretation by GO Enrichment
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