Partek Flow Documentation

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This tutorial gives an overview of RNA-Seq analysis with Partek® Flow®. It will guide you through an RNA-Seq workflow and result in the creation of a list of differentially expressed genes and subsequent visualisation of gene signatures by hierarchical clustering and interpretation of the list by gene ontology (GO) enrichment.

 Specifically, this tutorial will illustrate how to:

References

  1. Martin M. Cutadapt removes adapter sequences from high-throughput sequencing reads. EMBnet.journal 2011;17. Available at: http://journal.embnet.org/index.php/embnetjournal/article/view/200/458. Date accessed: 7 Apr 2016
  2. Benjamini Y, Hochberg Y. Controlling the false discovery rate: a practical and powerful approach to multiple testing. J Roy Stat Soc 1995;57:289-300.
  3. Xing Y, Yu T, Wu YN, Roy M, Kim J, Lee C. An expectation-maximization algorithm for probabilistic reconstructions of full-length isoforms from splice graphs. Nucleic Acids Res. 2006;34:3150-3160.
  4. Trapnell C, Williams BA, Pertea G, et al. Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation. Nat Biotechnol. 2010;28:511-515.

 

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2 Comments

  1. We should put the references on the individual child pages in which they are mentioned.